Journal: The Scientific World Journal

Article Title: A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries

doi: 10.1155/2013/460202

Figure Lengend Snippet: (a) To confirm that gcrR was deleted, chromosomal DNA was isolated from the selected transformants as well as UA159 progenitor and used as a template for PCR amplification with the P1/P2 and RT-gcrRF/RT-gcrRR primers. The resulting amplicons (the gcrR upstream-kanamycin resistance (KanR) cassette junction and the part of gcrR gene) were analyzed via agarose gel electrophoresis. Nucleotide sequencing of the purified amplicons with the same primer set was conducted to confirm the gcrR knockout and KanR cassette insertion. (b) Lane 1: the resulting amplicons (chromosomal DNA of MS-gcrR-def as template and RT-gcrRF/RT-gcrRR as primers); lane 2: the resulting amplicons (chromosomal DNA of UA159 as template and RT-gcrRF/RT-gcrRR as primers); lane 3: 1 kb marker (Fermentas). (c) Lanes 1, 2, 3, and 4: the resulting amplicons (chromosomal DNA of MS-gcrR-def as template and P1/P2 as primers); Lane 5, 1 kb marker (Fermentas); Lane 6, the resulting amplicons (chromosomal DNA of UA159 as template and P1/P2 as primers).

Article Snippet: Standard curves were generated for each primer-template set with a series of standard S. mutans UA159 genomic DNAs (ATCC, USA) that were diluted 10-fold.

Techniques: Isolation, Amplification, Agarose Gel Electrophoresis, Sequencing, Purification, Knock-Out, Marker

Journal: The Scientific World Journal

Article Title: A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries

doi: 10.1155/2013/460202

Figure Lengend Snippet: The adhesion ability of Streptococcus mutans UA159 as well as the MS-gcrR-def was determined based on the absorbance of the cells on the plate. *Significant differences of adhesion between UA159 and MS-gcrR-def ( P < 0.05) were found, while there was no variance in cell growth.

Article Snippet: Standard curves were generated for each primer-template set with a series of standard S. mutans UA159 genomic DNAs (ATCC, USA) that were diluted 10-fold.

Techniques:

Journal: The Scientific World Journal

Article Title: A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries

doi: 10.1155/2013/460202

Figure Lengend Snippet: (a) IOD (integrated optical density) of the green and red fluorescence from biofilm cells. (b) Biofilm thickness. The Z section was used to record the biofilm thickness. Vertical lines were chosen randomly to analyze each image. The red and green fluorescence intensities of the dead cells and living cells, respectively, were also quantified using Image Pro-plus. *Significant difference was found between green fluorescence of UA159 and MS-gcrR-def ( P < 0.05).

Article Snippet: Standard curves were generated for each primer-template set with a series of standard S. mutans UA159 genomic DNAs (ATCC, USA) that were diluted 10-fold.

Techniques: Fluorescence

Journal: The Scientific World Journal

Article Title: A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries

doi: 10.1155/2013/460202

Figure Lengend Snippet: For in vitro acid production, the initial pH of the culture was measured at 7.3 by using a pH meter. Each sample was repeated three times. *Significant differences were found between UA159 and MS-gcrR-def ( P < 0.05).

Article Snippet: Standard curves were generated for each primer-template set with a series of standard S. mutans UA159 genomic DNAs (ATCC, USA) that were diluted 10-fold.

Techniques: In Vitro

Journal: The Scientific World Journal

Article Title: A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries

doi: 10.1155/2013/460202

Figure Lengend Snippet: The enamel and dentinal caries scores of rats infected with UA159, MS-gcrR-def, and the mixture of both. The caries levels were scored by Keyes' method. The results are expressed as mean ± S.D. values. Symbols for statistical significance: *significantly different from the group UA159 infection alone ( P < 0.05).

Article Snippet: Standard curves were generated for each primer-template set with a series of standard S. mutans UA159 genomic DNAs (ATCC, USA) that were diluted 10-fold.

Techniques: Infection